![]() ![]() Equilibration of wells in the filter plates was performed by addition of 200 μL of loading buffer per well followed by agitation at 1100 rpm for 1 min, after which the buffer was removed by vacuum extraction. Static binding capacity (SBC) was determined in 6 μL PreDictor™ 96-well filter plates. Some characteristics of the MAbs are shown in Table 1. The two MAbs used in this study were initially purified from CHO cell supernatant by protein A affinity chromatography. The studies include measurement of static- and dynamic binding capacities at various binding conditions, as well as screening and optimization of gradient- and step-elution conditions.ġ Capto adhere ImpRes is also be used for purification of recombinant proteins and other biomolecules. This application note describes development of polishing steps for two different MAbs in B/E mode using Capto adhere ImpRes. Contaminants such as DNA, HCP, leached protein A, aggregates, and viruses are efficiently separated from monomeric MAbs1 in B/E or FT modes. The high resolution possible with Capto adhere ImpRes enables reduced buffer consumption and improved product yield compared with Capto adhere, a related product with the same ligand but with a larger bead size. The small bead size of Capto adhere ImpRes enables high-resolution purification of target protein. The medium enables operation in either B/E or nonbinding (flowthrough, FT) modes and results in either two- or three-step purification schemes. One of these options, Capto adhere ImpRes, is a cost-effective and flexible chromatography medium (resin) designed for high-resolution polishing of MAbs.Ĭapto adhere ImpRes is a multimodal anion exchange medium with a ligand (Fig 1) that displays high selectivity compared with traditional ion exchange polishing media. After the initial protein A capture step, there is a wide range of options for intermediate and polishing purification steps. ![]() GE Healthcare Life Sciences’ MAb production toolbox employs protein A chromatography media such as MabSelect SuRe™ or MabSelect SuRe LX for capture of the target. A platform approach is desirable as it saves both time and money in process development. The relative homogeneity of MAbs makes them well-suited for platform processes, which are sets of unit operations, conditions, and methods applied to molecules of a given class. As a result, more cost-effective, efficient, and flexible process purification schemes are one of the highest priorities for MAb manufacturers. ![]() MAbs and MAb conjugates are today in great demand for use as biopharamaceuticals. The results showed high yields of monomeric MAb, as well as good clearance of aggregates, host cell proteins (HCP), and leached protein A. The effects of buffer, pH, conductivity, and sample load were investigated. The study presents results from optimization of the loading conditions using the Design of Experiments (DoE) approach. In this study, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute (B/E) mode was evaluated. One continuous piece of Pittards® high-quality leather is applied directly to the palm to provide a reactive fit and feel.įasten the cinch cord securely and you’re ready to dive in.Capto adhere ImpRes is a strong ion exchanger with multimodal functionality designed for polishing of monoclonal antibodies (MAbs). The top of the hand is shielded by 40 grams of Thinsulate™ insulation and the same 45,000mm Sympatex® Membrane found in all our 2.0 outerwear, making the Capto Gauntlet completely waterproof and windproof. Whether you’re up to your ankles or your shoulders, the Gauntlet will not allow any snow, sleet, ice or rain anywhere near your hands. Ever heard the phrase “in too deep?” With the Capto Gauntlet, this phrase simply does not apply. ![]()
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![]() Hamster toys are great fun for your furry and furry little friend, but they can be expensive. In just 5 simple steps, you'll have an exciting plaything that provides exercise, exploration, and fun for your furry friend! Get your hammy going with this easy project now! How to Build Hamster Toys out of Household Items You'll love creating this DIY hamster toy that can be customized to fit your hamster's needs. Designed by animal lovers, these DIY hamster toys are easy to assemble, and your pet is sure to enjoy them. Transform a tissue box and some household tools into an awesome hamster toy. It's elementary to make and does not require much money or time. This video shows you the steps to creating a fun and simple toy that your pet will love. You can make a DIY hamster cage toy yourself. All you need is some leftover materials from around the house to create these creative hamster toys for your little friend! Please make hamster toys using standard household supplies and hang them around in the cage or playpen. Your pet can have fun inside this box hideout or climb up and down the wall on this climbing platform. You can make hamster toys with a few pieces of cardboard and some colorful non-toxic paint. These toys are great for beginner hamster owners because they are easy to make and the supplies needed can be found around your house! We will also cover the importance of a toy for your hamster, and how it can help them keep occupied and stimulated - making that bad behavior less likely to happen. In this video, I will show you how to make 2 Easy DIY Hamster Toys. They will love running around in it because since it's made from the Pringles can and cardboard, it's incredibly smooth! Easy To DIY Hamster Toys This is a great hamster toy that requires very few materials and can be folded up neatly when it is not in use, making it easy to store. Make a hamster roller with Pringles can and any paint. These are simple and easy to make, yet entertaining for your hamster for hours. These interactive toys are easy and fun to make, keeping your hamster amused while you're away at work. All you will need is some plastic bowl, cardboard, and a few other items. This video tutorial demonstrates how to make three easy boredom breakers for your hamster. Get creative with your pet and make a cute toy for them to play in. Squeaksandnibbles DIY Hamster Toys Tutorial It will keep your pet healthy and occupied when you're away from home. This DIY project is easy to put together and can be used as an everyday toy or a plaything for holidays and special occasions. You can make them at home for a fraction of store-bought toys. They need toys that let them exercise their jaws and keep their teeth healthy. Hamsters love to chew, and their teeth grow constantly. Tie a knot at one end of an old shoelace to create a noose that's easy for your hamster to grab onto (it's also great for hanging from the top of their cage).This will give your hamster something to climb on and explore! If you have a wire cage, try threading some string through all of the bars along one wall, then attaching it to different toys.Add a loop of string or wire at one end of the toy so you can hang it on the side of the cage.This will make it easier for your hamster to find the toy in their cage. Now that you have the basics down, let's get creative! Here are some great tips and tricks to help you make your DIY hamster toys even more fun.Cardboard (this can be from cereal boxes or shoe boxes). ![]() You'll be amazed at how easy they are to make but even more amazed by your little furry friend's happy face when they see what new game you've made for them! Materials You Need For DIY Hamster Toys So if you love your little furry friend as much as we do, scroll down and get inspired by these brilliant DIY hamster toys. There are plenty of items that make excellent playthings, and there are even some easy options as well. We've picked the best DIY Hamster Toy Ideas from around the web. ![]() This post is for you if you're looking for creative hamster toys. Here are some tips if you are looking for a way to make hamster toys. These little rodents enjoy playing and exploring, so it is essential to provide them with toys that will keep them occupied. Hamsters are fun pets that are easy to care for and can be kept in small cages. Many projects are quick and inexpensive, so they're great for busy pet parents who don't have much spare time to create homemade hamster toys. These DIY Hamster Toy Ideas offer hours of entertainment for your pet and will keep him from getting bored. Hamsters need a lot of toys to keep them entertained otherwise, they will find trouble on their own. They can make excellent pets for children, but knowing how to take care of them is essential. Hamsters are cute, cuddly, and very curious. ![]() ![]() ![]() Free Online GIF Maker to Create a GIF on iPhoneīesides the two methods above, you can rely on some free GIF makers online to create animated GIFs on your iOS device. If you want to store this created GIF on your iPhone, you can tap the Share GIF button and then choose Save GIF. Here you can choose to Share GIF or Upload to GIPHY based on your need. Tap the purple arrow in the bottom-right corner to enter its Upload interface. This iPhone GIF maker app offers various options on the bottom. Step 3 After loading your video clip to GIPHY, you can freely edit and decorate it with animated stickers, text, filters, and more. Here we take making a video into a GIF as an example. You can directly record a new video clip or create a GIF from Photos. Then you are offered two options to make a GIF on your iPhone. Step 2 Tap the Create button in the top-right corner and tap Continue to allow the Camera Access. You are allowed to sign in directly with your Facebook or Apple account. Step 1 Open the GIPHY app on your iPhone and sign up for an account. You can free install it on your iPhone and then follow the steps below to make videos into GIFs on your iPhone. It also enables you to edit and create GIFs with ease. It collects lots of GIFs in different categories for you to share or download. Here we recommend the popular GIF maker app, GIPHY, to make GIFs on iPhone. You can easily find many GIF maker apps from App Store to turn video clips or pictures into GIFs. Best GIF Maker App for iPhone to Create Animated GIFs You can also use Bounce to make the live photo frames go forward and reverse.įree Download Free Download Part 2. ![]() Here you should select Loop to convert your live photo to a repeating GIF file. Tap the LIVE button in the upper-left corner to bring up more moving picture options, Live, Loop, Bounce, Long Exposure, and Off. Step 2 Choose the live photo you want to turn into a GIF. Tap Albums on the bottom screen, then scroll down to enter Live Photos. Step 1 Turn on your iPhone and open the Photos app. Now you can take the following guide to turn live photos into GIFs. Making a live photo repeat can be the easiest way to create a GIF on iPhone. Live Photos is a built-in IOS feature that allows you to capture a couple of seconds of animation. You can use Live Photos in the Photos app when you want to make a GIF on your iPhone. How to Make GIFs on an iPhone with Live Photos GIF Maker to Create a GIF Online on iPhone Best GIF Maker App for iPhone to Create GIFs ![]() ![]() ![]() Within the period, you have the credit of 150 outbound fax pages and 200 inbound fax pages. This online fax service provides you with the facility of free 30-day fax trial. One Fax Number can be linked with up to 5 email addresses, which is a considerate feature. Signup is required so that you will be assigned a Fax Number as the credential of sending and receiving faxes. It enables users to send and receive faxes by email, which is very handy to get notifications for daily use. MyFax features with two-way online fax service. ![]() MyFax – Online Fax Service as Easy as Sending/Receiving Email Anyway, it would be a very convenient fax service available for all platforms if you’re read to pay for long-term use. Send to multiple fax recipients in one fax.Īccording to the feedback of users, this online fax service does not provide valid free trial.Professional and Basic Plans include personal fax number.Directly access Dropbox, Box, Google Drive and OneDrive.Available for Web, Microsoft Windows, Mac OS X, iPhone/iPad, Android and Blackberry.Low pricing per page starting at $0.11.On the one hand, PamFax is able to satisfy you for almost all needs of sending/receiving faxes online, on the other hand, however, it is not an easy tool to deal with since signup is compulsive. PamFax – All-in-one Multi-platform Online Fax Serviceĭifferent from the simplicity of FaxZero, PamFax has been developed to be a comprehensive and sophisticated online fax service. ![]() In case you lose important data, make sure to try iReparo for PC to get back your files as soon as possible. Pro tips: Don’t delete the original copy immediately after faxing the files and documents. Once the fax is sent, you will be given a URL which will show the status of the fax.
![]() ![]() You start off with the basic and then as you progress, you will earn money to buy yourself a much improved rifle. Be sure to scroll through as there are lots of different options! Then select the weapon icon, here you can choose your weapon and purchase a new one. How to Play: On the home page, select the arrows to choose the environment you play in. Try and eliminate the target in one shot! Once spotted it is up to you to be accurate and aim for them. This game will develop your observational skills as you must search through your scope for your targets. ![]() ![]() Do this by firing your rifle and you will get a close up slow mo of the impact if you hit your target. The aim of the game is to eliminate everyone you see. Play this exciting game and experience what life is really like for a sniper marksman! Once the targets have been dispatched, the mission will end and you will progress. You are a marksman sniper and you must use your abilities to get the job complete. You even can download the application to install it on your mobile phone to play it offline, while not having an internet connection.Sniper 3D is a fast-paced and addicting shooting game where you must eliminate all the targets. You can play the Run 3 Unblocked via your Web browser, Android or iOS. So, unique graphics are going to combine the 3D gameplay with the cartoons, to amuse you to own you and to give you a greater style of playing the game and much more. You will get the new character of an alien with greater ability to sustain in that situation and help you survive for the longer period of the time. So, tap to change the alien or characteristics of looks and shape of that alien. You can find new alien characters in the game if you do not like the aliens running around to avoid falling down in the holes. There are no greater chances of your survival, but you to survive to cross the endless battle of running and jumping. There would be great obstacles there you can change the gravity by walking or running along the walls. You have to run to cross all the hurdles which are coming to stop you. If you are already expert and want to test your knowledge then just start playing! Cool Math Games Run Run 3 unblocked games You are going to have a great fun via Run 3 online unblocked game, just tap and run into a valley. They are easy to play anywhere you want and also helps in increased exposure. Pre-Algebra Lessons School students can get expertise in Algebra with Cool Math games Online. The tips and tricks of finding a solution to mathematical problems are available in these memory games. With the help of characters and subjects in a game, you can find logics for your next mathematical assignment Number Games No one can forget number with these math playground available online! If you want to see how fast you can count or how much you are knowledgeable with numbers, then just start your gameplay! cool math games run These games have certain storyboards and scenes that will always help in memorizing difficult facts. ![]() How To Play Favorite Cool Math Games? Here are some of the exciting options for you to start a better game play according to your choice! Logic games Playing Cool math unblocked games online can help in finding deductions for certain mathematical problems. ![]() ![]() If one of the partners has displayed cards on the table opening the game, their partner may, in turn, display their melds or add cards to the melds already shown, without having to comply with this opening requirement. Having Barbele makes it difficult for players who have reached the said score to meld their initial cards for each partial game, since they must have a meld with a minimum value of 70 points in order to be able to display cards for the first time. A partial game can also end because there are no cards left in the stock deck, although this very rarely happens.Ī player or couple is said to have Barbele when, at the end of a partial game, their score reaches or exceeds 750 points. When a player says “Pumba” and there is only one discard left on the table, they cannot take it to finish the game, there must be at least two discards on the table so in this case they will have to take a card from the stock deck. When, after discarding, a player only has one card left in their hand, they must warn both their partner and the opposing players that they are about to finish by saying “Pumba” out loud. If this is done in the first turn of the game, it is called “Folding the hand”, i the hope that it makes it easier for their partner to play all of their cards. Folding is finishing a partial game by showing all the cards in a hand at once. If the player still has one card left, they can also end the game by discarding it. ![]() The cards must be place face up on the table in front of only one of the team, since the game is common to both, and in such a way that all the card numbers are visible so that the melds, especially straights, are clearly visible.Īny player can end and win a hand in their turn when they get rid of all the cards in their hand, placing them face up on the table in one or more combinations or adding them to those already on show. This discard is always done using a card being held, never with one already face up on the table. The turn ends by having to discard a card. Showing cards or combinations is not obligatory, it can be done in a later turn when more advantageous. Next, the player shows their cards in minimum combinations of three cards, either in sets of equal cards or in straights, using as many wild cards as they please or adding cards to the their partner’s previous melds. The game continues, strictly from left to right, and each player in turn must take the top card off the deck or the discards that are left face up on the table, bearing in mind that they have to take all of the discards, even if they are only interested in one of them. If the player has melded a combination in their hand they can choose to show it, ending their turn by discarding a card, which is left face up on the table. ![]() The “hand” starts by choosing to take either the top card off the deck or the upturned card on the table. ![]() ![]() If you need detailed instructions, follow the guidance text. There is a setup DMG file for MacOS Ventura and Monterey 2023. You do not have to browse through multiple torrent trackers to find exactly what you need.Ĭlick the button below to get Folx Pro v5.27 MacOS Free Download link full features. You will benefit from an amazing feature in the PRO version of Folx – search for torrents directly from Folx’s interface. Thanks to its numerous useful settings and unique system of sorting content, it offers complete control over the download process. How to Install Folx Pro v5.27 Mac Full Crackįolx is a powerful and easy-to-use Mac download manager that is fully compatible with the latest macOS Monterey and Ventura. Gives you complete control over the downloading speed of your Mac.Folx Mac downloader allows downloading files at specific times.Password saver for fast access to your favorite websites.Its auto-resume option comes handy when the downloading gets interrupted. ![]()
![]() ![]() She holds an MA in Spiritual Formation and is the author of "The Wild Land Within" published with Broadleaf Books.A circular pattie with a soft mint center coated in dark chocolate. Lisa Colón DeLay is a writer, designer, teacher, speaker, and host of the weekly broadcast Spark My Muse. Lisa, peppermint patty lover, God lover, and putting on the pounds with various chocolates. Your thoughts and comments are always welcome. If you had fun, got a laugh, were the least bit amused, or even got mad, please tell a few people to drop by and visit to take the self-test for themselves. ![]() And also, the test really isn’t scientific, in case you were wondering. Okay, I’m not really sure about that last part, but I do think something could go wrong in this whole setup, I’m just not completely sure what. On the other hand, you could get terribly fat filling a huge spiritual void with empty calories, and go to hell in the process. ![]() It may help integrate your spirituality and draw you into a more intimate relationship with God, in everyday ways. If you got more than 5 True answers you have made an irrevocable connection between a minty chocolate treat and one, or more, of the three persons of God. You are finding that something simple like a York Peppermint Patty can somehow remind you of God, and yet make you a bit hungry for candy at the same time. Maybe you’d look at him in the eyes to check for a “knowing look,” after you averted eye contact for a little bit, first.)ġ0. After realizing that he would know anyways you would hope he wouldn’t mind if you kept the whole thing. If you had one, you would split it, but maybe you would have a brief conflict of conscience thinking of hiding it, or not mentioning it. (Perhaps you can relate to this scenario: If you had two, and if he was physically there, you would give him one. You can imagine Jesus eating and enjoying a York Peppermint Patty. If it were not sacrilegious, or highly odd, you could imagine the Holy Ghost (or Spirit) as a good choice for the pitchman for York Peppermint Patty and saying, “Get the (cool) sensation,” in a dry humor sort of way.ĩ. The idea of dressing up as the Holy Ghost has never crossed your mind, yet you realize drinking some milk when eating chocolate makes sense.Ĩ. If chocolate was served instead of bread at Communion, you would look forward to going to church more often.ħ. You like to say “God bless you” when others sneeze–and now that you think of it, a dry and powerful sneeze seems practically other-worldly. Watching shows like Ghost Hunters is fairly tempting.ĥ. When eating minty candy, sometimes you feel a cool draft, or get a bit of a chill.Ĥ. Spiritual things are sometimes what you would call “spine-tingling”.ģ. When you hear the word “Holy Ghost” you get “shivers.” (You prefer the less weird term “Holy Spirit.”)Ģ. Some of you won’t remember the goofy York Peppermint Patty commercials of long ago, but thanks to the marvel of technology, you can view a couple retro ad pieces right now to either refresh your memory, or thank God you were not routinely subjected to such silliness.Īfter you watch the short video, have some fun and take this self-test to see how you score.Īnswer true or false, and add up your scores.ġ. Self-test: Is the Holy Spirit like a York Peppermint Patty? ![]() ![]() The cookies is used to store the user consent for the cookies in the category "Necessary". This cookie is set by GDPR Cookie Consent plugin. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. Nevertheless, I did giggle at the Rocky-like training scene, brought to you by John the penis.
![]() ![]() i long to voice out my opinion, even if no one can hear me. this year, i plan to make it fun, to write about topics i’m genuinely interested in. blogging was not fun for me nor was it rewarding. i’ve realized that i was publishing things i did not like doing. the reason why i avoided it was because i disliked the way i did it. blogging, i found, is something very dear to my heart. still, what matters is i noticed it and it did bother me. the wordpress even changed layout while i was goneĭon’t worry, there’s a huge chance none of you noticed this. i had not pressed the button to create a new post for a long time. there were no pings in emails indicating i had published. it was written for the sake of getting it done, so it could also count as a nonexistent post. the two recent posts, a interview with an author, was missing so much passion and dedication present in previous articles. it was a wasteland of old reviews, wrap ups, abandoned. I recommend Kung Fu Panda to anyone who wants to watch a light comedy yet still discover deep realizations, as what i have experienced.įor five months, i had disappeared. as for me, i’m not sure i can respond it that confidently but i have a inkling to what the answer is. po likes to ask, “who am i?” and it is a monster of a question most people would ask themselves for years. In every Kung Fu Panda movie i’ve watched, the concept of “self” is always discussed. they remind me that talent is useful but passion and the drive to do it is much more enriching. these movies remind me, weirdly, of writing, even if it doesn’t have to do anything with it. when someone so enthusiastic about something, i can’t help but feel excited back. the passion and dedication, not to mention perseverance and determination of someone doesn’t only benefit them, it also affects those around them. (i guess it’s one of the most used tropes and overdone messages a form of art can deliver but i like this so much better than the “true love” thing) i always get so inspired to write when i see it. I like the “do your best, don’t quit, hard work will pay off” movies, wherein the protagonist decides to do something he/she is so passionate about. and seeing it in po and Master Oogway, i am just in awe. to be humble and to be wise are what i strive for in life. i guess you can say humility is somehow wisdom, for those two are the hardest titles to claim. the humble are far greater than the most skilled. Po is my favorite of the bunch, followed closely by Master Oogway. it is difficult to push past this level and all those who succeed, i greatly admire. ![]() the absence of support and faith of the people around you weigh you down. you know you can do it and you’re trying but people can’t seem to see it. ![]() as a human, i know how it feels like to be “less”, although not as extreme as po’s. the ones he dreamed meeting, ones supposed to be the model of humility and respect, denied him because of his lack of “skills”. you can see the immense disappointment and loss of self esteem in his chubby face. when (spoilers) Po was rejected by the heroes he looked up on and the master destined to train him, i was holding back my tears. Kung Fu Panda brought tears to my eyes, especially Kung Fu Panda the first. i’d like to talk more about the first and the third. it is something, as one movie* stated, you pursue.Ĭurrently, there are three movies in this series. nevertheless, i do think it’s a nice message to, not only kids but adults, that happiness is attainable. is happiness really that impossible, something to roll our eyes at when stories present it to us? i know that everything doesn’t end happily. people like to say that, don’t they? when a story ends with a smile, these people raise their eyebrows and declare, “unrealistic.” to be honest, i don’t think it that way. a child’s movie, one with a happy ending to apparently “mask the harsh realities of the world”. Kung fu panda 3 has just been recently released. a stupid thought that you should never ponder about. i guess i thought that the world didn’t need my opinion. how could i be more random and out of the blue? still, i’ve been meaning to write more reflections about the movies i’ve watched, an action i refused, for some reason, to do long ago. I undestand this comes as a shock to you. ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |