![]() ![]() Equilibration of wells in the filter plates was performed by addition of 200 μL of loading buffer per well followed by agitation at 1100 rpm for 1 min, after which the buffer was removed by vacuum extraction. Static binding capacity (SBC) was determined in 6 μL PreDictor™ 96-well filter plates. Some characteristics of the MAbs are shown in Table 1. The two MAbs used in this study were initially purified from CHO cell supernatant by protein A affinity chromatography. The studies include measurement of static- and dynamic binding capacities at various binding conditions, as well as screening and optimization of gradient- and step-elution conditions.ġ Capto adhere ImpRes is also be used for purification of recombinant proteins and other biomolecules. This application note describes development of polishing steps for two different MAbs in B/E mode using Capto adhere ImpRes. Contaminants such as DNA, HCP, leached protein A, aggregates, and viruses are efficiently separated from monomeric MAbs1 in B/E or FT modes. The high resolution possible with Capto adhere ImpRes enables reduced buffer consumption and improved product yield compared with Capto adhere, a related product with the same ligand but with a larger bead size. The small bead size of Capto adhere ImpRes enables high-resolution purification of target protein. The medium enables operation in either B/E or nonbinding (flowthrough, FT) modes and results in either two- or three-step purification schemes. One of these options, Capto adhere ImpRes, is a cost-effective and flexible chromatography medium (resin) designed for high-resolution polishing of MAbs.Ĭapto adhere ImpRes is a multimodal anion exchange medium with a ligand (Fig 1) that displays high selectivity compared with traditional ion exchange polishing media. After the initial protein A capture step, there is a wide range of options for intermediate and polishing purification steps. ![]() GE Healthcare Life Sciences’ MAb production toolbox employs protein A chromatography media such as MabSelect SuRe™ or MabSelect SuRe LX for capture of the target. A platform approach is desirable as it saves both time and money in process development. The relative homogeneity of MAbs makes them well-suited for platform processes, which are sets of unit operations, conditions, and methods applied to molecules of a given class. As a result, more cost-effective, efficient, and flexible process purification schemes are one of the highest priorities for MAb manufacturers. ![]() MAbs and MAb conjugates are today in great demand for use as biopharamaceuticals. The results showed high yields of monomeric MAb, as well as good clearance of aggregates, host cell proteins (HCP), and leached protein A. The effects of buffer, pH, conductivity, and sample load were investigated. The study presents results from optimization of the loading conditions using the Design of Experiments (DoE) approach. In this study, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute (B/E) mode was evaluated. One continuous piece of Pittards® high-quality leather is applied directly to the palm to provide a reactive fit and feel.įasten the cinch cord securely and you’re ready to dive in.Capto adhere ImpRes is a strong ion exchanger with multimodal functionality designed for polishing of monoclonal antibodies (MAbs). The top of the hand is shielded by 40 grams of Thinsulate™ insulation and the same 45,000mm Sympatex® Membrane found in all our 2.0 outerwear, making the Capto Gauntlet completely waterproof and windproof. Whether you’re up to your ankles or your shoulders, the Gauntlet will not allow any snow, sleet, ice or rain anywhere near your hands. Ever heard the phrase “in too deep?” With the Capto Gauntlet, this phrase simply does not apply. ![]()
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